验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19321-1-AP targets H1FX in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5819 Product name: Recombinant human H1FX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC000426 Sequence: MSVELEEALPVTTAEGMAKKVTKAGGSAALSPSKKRKNSKKKNQPGKYSQLVVETIRRLGERNGSSLAKIYTEAKKVPWFDQQNGRTYLKYSIKALVQNDTLLQVKGTGANGSFKLNRKKLEGGGERRGAPAAATAPAPTAHKAKKAAPGAAGSRRADKKPARGQKPEQRSHKKGAGAKKDKGGKAKKTAAAGGKKVKKAAKPSVPKVPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | H1 histone family, member X |
| 别名 | Histone H1.10, Histone H1, Histone, H1X, H1-10 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC000426 |
| 基因名称 | H1FX |
| Gene ID (NCBI) | 8971 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92522 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
H1FX (Histone H1x) belongs to the histone H1/H5 family which plays a crucial role in the organization and regulation of chromatin structure.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for H1FX antibody 19321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
