验证数据展示
经过测试的应用
| Positive WB detected in | EL-4 cells, EL-4-B5 cells, NIH/3T3 cells, mouse liver tissue |
| Positive IP detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30695-1-AP targets GSDMD in WB, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 | GSDMD fusion protein Ag33609 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gasdermin D |
| 别名 | M2 4, Gsdmdc1, gasdermin D, Dfna5l, DF5L |
| 计算分子量 | 53 kDa |
| 观测分子量 | 50-53 kDa, 43 kDa |
| GenBank蛋白编号 | BC029813 |
| 基因名称 | Gsdmd |
| Gene ID (NCBI) | 69146 |
| RRID | AB_3086391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9D8T2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. This antibody can detect 53 kDa full-length GSDMD and 43 kDa fragment as described in the literature (PMID: 28392147, PMID: 28979266).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSDMD antibody 30695-1-AP | Download protocol |
| IP protocol for GSDMD antibody 30695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
