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验证数据展示

  • WB analysis of human placenta using 27079-1-AP

    WB analysis of human placenta using 27079-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27079-1-AP (GH1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary adenoma using 27079-1-AP

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary adenoma using 27079-1-AP

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Growth Hormone Polyclonal antibody

Growth Hormone Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27079-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GH, GH1, growth hormone 1, GH-N, GH 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pituitary adenoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27079-1-AP targets Growth Hormone in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22841

Product name: Recombinant human GH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 18-98 aa of BC075012

Sequence: LPWLQEGSAFPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GH1
别名 GH, GH1, growth hormone 1, GH-N, GH 1
计算分子量 217 aa, 25 kDa
观测分子量 22 kDa
GenBank蛋白编号BC075012
基因名称 GH1
Gene ID (NCBI) 2688
RRIDAB_2880745
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GH1, also named as GH or GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

实验方案

Product Specific Protocols
IHC protocol for Growth Hormone antibody 27079-1-APDownload protocol
WB protocol for Growth Hormone antibody 27079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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