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验证数据展示

  • FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were stained with APC Anti-Human CD8a before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98070-1-RR

    Affinity and Kinetic Characterization of 98070-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

Anti-Human Granzyme B Rabbit Recombinant Antibody, PBS Only

Granzyme B Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98070-1-PBS

产品说明书

CloneNo. 240761G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

GZMB, CSP B, CGL1, CGL 1, CCPI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98070-1-PBS targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
别名 GZMB, CSP B, CGL1, CGL 1, CCPI
计算分子量 247 aa, 28 kDa
GenBank蛋白编号BC030195
基因名称 Granzyme B
Gene ID (NCBI) 3002
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10144
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces lamin B degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full-length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32 kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.