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验证数据展示

  • WB analysis using 29491-1-AP

    WB analysis using 29491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29491-1-AP

    WB analysis using 29491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Progranulin/PGRN Polyclonal antibody

Progranulin/PGRN Polyclonal Antibody for WB, ELISA
Cat No. 29491-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, HEK-293 cells, HepG2 cells, MCF-7 cells, U-937 cells, J774A.1 cells, NIH/3T3 cells, RAW 264.7 cells, mouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy GRN Ready-To-Use IHC Kit. GRN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29491-1-AP targets Progranulin/PGRN in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Progranulin/PGRN fusion protein Ag30723 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 granulin
别名 granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88
计算分子量 64 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC000324
基因名称 Granulin
Gene ID (NCBI) 2896
RRIDAB_3086138
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28799
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

实验方案

Product Specific Protocols
WB protocol for Progranulin/PGRN antibody 29491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols