验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, TT cells, HuH-7 cells, mouse cerebellum tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87773-1-RR targets Gpr44 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 44 |
| 别名 | CD294, Chemoattractant receptor-homologous molecule expressed on Th2 cells, Crth2, G-protein coupled receptor 44, Prostaglandin D2 receptor 2 |
| 观测分子量 | 35 kDa, 40 kDa |
| GenBank蛋白编号 | BC111906 |
| 基因名称 | Gpr44 |
| Gene ID (NCBI) | 14764 |
| RRID | AB_3745746 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Z2J6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR44 (PTGDR2/CRTH2) is a Gi-coupled prostaglandin D₂ receptor expressed on Th2 lymphocytes, eosinophils, and basophils, where it mediates chemotaxis and activation of type 2 immune cells. Through PGD₂-dependent signaling, GPR44 plays a central role in allergic inflammation and eosinophilic immune responses, making it an important therapeutic target in asthma and other Th2-mediated diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Gpr44 antibody 87773-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
