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验证数据展示

  • WB analysis of RAW 264.7 using 86874-1-RR (same clone as 86874-1-PBS)

    WB analysis of RAW 264.7 using 86874-1-RR (same clone as 86874-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

    IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

    IHC staining of mouse heart using 86874-1-RR (same clone as 86874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86874-1-RR (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skin using 86874-1-RR (same clone as 86874-1-PBS)

    IP experiment of mouse skin using 86874-1-RR (same clone as 86874-1-PBS)

    IP result of anti-GPNMB (IP:86874-1-RR, 4ug; Detection:86874-1-RR 1:1500) with mouse skin tissue lysate 1120 ug. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02737-1

    Sandwich ELISA sample test of MP02737-1

    Serum of six mice was measured. The GPNMB concentration of detected samples was determined to be 14.00 ng/mL with a range of 10.09-19.47 ng/mL.

  • Sandwich ELISA standard curve of MP02737-1

    Sandwich ELISA standard curve of MP02737-1

    Sandwich ELISA standard curve of MP02737-1, Mouse GPNMB Recombinant Matched Antibody Pair - PBS only. 86874-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2814. 86874-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

Mouse GPNMB Recombinant monoclonal antibody, PBS Only (Capture)

GPNMB Uni-rAb® Recombinant Antibody for WB, IHC, IP, Sandwich ELISA, Indirect ELISA
Cat No. 86874-1-PBS

产品说明书

CloneNo. 250309C11

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IP, Sandwich ELISA, Indirect ELISA

别名

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86874-1-PBS targets GPNMB as part of a matched antibody pair:

MP02737-1: 86874-1-PBS capture and 86874-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IP, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2814

Product name: Recombinant Mouse GPNMB protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 23-502 aa of NM_053110.4

Sequence: KRFRDVLGHEQYPDHMREHNQLRGWSSDENEWDEHLYPVWRRGDGRWKDSWEGGRVQAVLTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYEKNCRNDLGLTSDLHVYNWTAGADDGDWEDGTSRSQHLRFPDRRPFPRPHGWKKWSFVYVFHTLGQYFQKLGRCSARVSINTVNLTAGPQVMEVTVFRRYGRAYIPISKVKDVYVITDQIPVFVTMSQKNDRNLSDEIFLRDLPIVFDVLIHDPSHFLNDSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNLNLTVQTAVPGPCPPPSPSTPPPPSTPPSPPPSPLPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVEGILEVSIMQIADVPMPTPQPANSLMDFTVTCKGATPMEACTIISDPTCQIAQNRVCSPVAVDGLCLLSVRRAFNGSGTYCVNFTLGDDASLALTSTLISIPGKDPDSPLRAVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycoprotein (transmembrane) nmb
别名 Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb
计算分子量64 kDa
观测分子量90-100 kDa, 120kDa
GenBank蛋白编号NM_053110.4
基因名称 Gpnmb
Gene ID (NCBI) 93695
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99P91
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

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