验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, L02 cells, MG-63 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21526-1-AP targets Glypican 6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag16135 Product name: Recombinant human GPC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-278 aa of BC106947 Sequence: RAFIAARTFVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYCLNVMKGCL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glypican 6 |
| 别名 | GPC6, Secreted glypican-6, UNQ369/PRO705 |
| 计算分子量 | 555 aa, 63 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC106947 |
| 基因名称 | GPC6 |
| Gene ID (NCBI) | 10082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y625 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glypican-6 (GPC6) is a glycosylphosphatidylinositol-anchored heparan-sulfate proteoglycan that regulates cell-surface signaling cues during development and disease. GPC6 binds Hedgehog ligands and facilitates their interaction with the receptor Patched, thereby amplifying Hh signaling required for long-bone and gastric/intestinal elongation. (PMID: 35235423)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Glypican 6 antibody 21526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
