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验证数据展示

  • WB analysis using 13521-1-AP (same clone as 13521-1-PBS)

    WB analysis using 13521-1-AP (same clone as 13521-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13521-1-AP (Glypican 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13521-1-PBS in a different storage buffer formulation.

  • WB analysis using 13521-1-AP (same clone as 13521-1-PBS)

    WB analysis using 13521-1-AP (same clone as 13521-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13521-1-AP (Glypican 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13521-1-PBS in a different storage buffer formulation.

Glypican 2 Polyclonal antibody, PBS Only

Glypican 2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 13521-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13521-1-PBS targets Glypican 2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4041

Product name: Recombinant human GPC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-332 aa of BC027972

Sequence: MSALRPLLLLLLPLCPGPGPGPGSEAKVTRSCAETRQVLGARGYSLNLIPPALISGEHLRVCPQEYTCCSSETEQRLIRETEATFRGLVEDSGSFLVHTLAARHRKFDEFFLEMLSVAQHSLTQLFSHSYGRLYAQHALIFNGLFSRLRDFYGESGEGLDDTLADFWAQLLERVFPLLHPQYSFPPDYLLCLSRLASSTDGSLQPFGDSPRRLRLQITRTLVAARAFVQGLETGRNVVSEALKVPVSEGCSQALMRLIGCPLCRGVPSLMPCQGFCLNVVRGCLSSRGLEPDWGNYLDGLLILADKLQGPFSFELTAESIGVKISEGLMYLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glypican 2
别名
计算分子量 579 aa, 63 kDa
观测分子量62，70-100 kDa
GenBank蛋白编号BC027972
基因名称 GPC2
Gene ID (NCBI) 221914
RRIDAB_3085417
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N158
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glypican 2 (GPC2) is a heparan sulfate proteoglycan that is linked to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage(PMID: 18505598). It is expected to be located in cell membrane and highly overexpressed in a high percentage of human neuroblastomas, medulloblastomas, and retinoblastomas. The calculated molecular weight of GPC2 is 62 kDa. And other bands are the result of glycosylation modification. Recent studies demonstrate that expression of the GPC2 protein is significantly increased in neuroblastoma and undetectable in normal tissues, including brain, heart, lung, and kidney, indicating that GPC2 is a suitable tumor antigen in neuroblastoma (PMID: 30352677).

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