验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, rat kidney tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86604-3-RR targets Glycerokinase in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4175 Product name: Recombinant human GK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC037549 Sequence: MAASKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEKTCEKLGQLNIDISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKQLCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAKEVGTSYG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glycerol kinase |
| 别名 | GK, ATP:glycerol 3-phosphotransferase, EC:2.7.1.30, GK1, GKD |
| 计算分子量 | 524 aa, 57 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC037549 |
| 基因名称 | Glycerokinase |
| Gene ID (NCBI) | 2710 |
| RRID | AB_3745000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P32189 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycerokinase, also known as glycerol kinase (GK, EC 2.7.1.30), is a widely conserved phosphotransferase that catalyzes the ATP-dependent phosphorylation of glycerol to generate sn-glycerol-3-phosphate (G3P). Glycerokinase exists as four isoforms with apparent molecular masses of 61 kDa, 57 kDa, 61 kDa, and 58 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Glycerokinase antibody 86604-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
