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GlyT2 Polyclonal antibody

GlyT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 25948-1-AP

Print datasheet

Synonyms

GlyT 2, GLYT2, NET1, SLC6A5

验证数据展示

  • WB analysis using 25948-1-AP

    WB analysis using 25948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25948-1-AP (GlyT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25948-1-AP targets GlyT2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity mouse, rat
Immunogen GlyT2 fusion protein Ag22835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms GlyT 2, GLYT2, NET1, SLC6A5
Calculated Molecular Weight 797 aa, 87 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC096319
Gene Symbol SLC6A5
Gene ID (NCBI) 9152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y345
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GlyT2 is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. Like other members of the family, GlyT2 is trafficked to the cell surface as homo-oligomers from the endoplasmic reticulum (ER) (PMID: 32714574). GlyT2 is expressed in medulla, and to a lesser extent in spinal cord and cerebellum. GlyT2 has 3 isoforms with the molecular mass of 63, 86 and 87 kDa. The 63 kDa band is the unglycosylated GlyT2 polypeptide, whereas the 70-110 kDa band is a differential glycosylation form of GlyT2 (PMID: 17851090, 17962467).

实验方案

Product Specific Protocols
WB protocol for GlyT2 antibody 25948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols