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Species Reactivity
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验证数据展示

  • WB analysis using 66904-1-Ig (same clone as 66904-1-PBS)

    WB analysis using 66904-1-Ig (same clone as 66904-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    IHC staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    IF Staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66904-1-PBS

    IF Staining of mouse brain using 66904-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glucocorticoid receptor antibody (66904-1-PBS, Clone: 1D9A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glucocorticoid receptor Monoclonal antibody, PBS Only

Glucocorticoid receptor Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66904-1-PBS

产品说明书

CloneNo. 1D9A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

NR3C1, 1D9A9, GCCR, GCR, GR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66904-1-PBS targets Glucocorticoid receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Glucocorticoid receptor fusion protein Ag28431 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 NR3C1, 1D9A9, GCCR, GCR, GR
计算分子量 86 kDa
观测分子量 97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2857354
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04150
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).