验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human stomach cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy GAST Ready-To-Use IHC Kit. GAST primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
60346-1-Ig targets Gastrin in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12795 Product name: Recombinant human GAST protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-101 aa of BC069724 Sequence: SEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | gastrin |
| 别名 | G14 Gastrin 6, G17 Gastrin 14, G34 Gastrin, G52 Big gastrin, G6, GAS, GAST, gastrin, Gastrin 17, Gastrin 34, Gastrin component I Gastrin 52, Gastrin component II, Gastrin component III |
| 计算分子量 | 101 aa, 11 kDa |
| GenBank蛋白编号 | BC069724 |
| 基因名称 | Gastrin |
| Gene ID (NCBI) | 2520 |
| RRID | AB_2881455 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01350 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gastrin is a hormone produced primarily by G-cells in the stomach, where it functions to stimulate acid secretion by gastric parietal cells. Gastrin is a promiscuous hormone. Thus, the gastrin gene is expressed in common cancers such as bronchogenic, colorectal, ovarian and pancreatic carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Gastrin antibody 60346-1-Ig | Download protocol |
| IHC protocol for Gastrin antibody 60346-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virchows Arch Mixed adenoma-neuroendocrine tumor of the stomach: analysis of nine cases with literature review |
