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验证数据展示

  • WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

    WB analysis using 60223-1-Ig (same clone as 60223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60223-1-Ig (same clone as 60223-1-PBS)

    WB analysis of HepG2 using 60223-1-Ig (same clone as 60223-1-PBS)

    WB result of Galectin-1 antibody (60223-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IF Staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS1 antibody included
  • IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    IHC staining of human colon cancer using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGALS1 antibody included
  • IF Staining of U2OS using 60223-1-Ig (same clone as 60223-1-PBS)

    IF Staining of U2OS using 60223-1-Ig (same clone as 60223-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Galectin-1 Monoclonal antibody, PBS Only

Galectin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60223-1-PBS

产品说明书

CloneNo. 3G10D2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

3G10D2, Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.

产品信息

60223-1-PBS targets Galectin-1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2432

Product name: Recombinant human Galectin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC020675

Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 1
别名 3G10D2, Beta-galactoside-binding lectin L-14-I, Gal 1, GAL1, Gal-1
计算分子量 15 kDa
观测分子量 13 kDa
GenBank蛋白编号BC020675
基因名称 Galectin-1
Gene ID (NCBI) 3956
RRIDAB_11182608
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09382
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

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