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验证数据展示

  • WB analysis using 31521-1-AP

    WB analysis using 31521-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31521-1-AP (GZMB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 31521-1-AP

    IHC staining of human lymphoma using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 31521-1-AP

    IHC staining of human lymphoma using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31521-1-AP

    IHC staining of human tonsillitis using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31521-1-AP

    IHC staining of human tonsillitis using 31521-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31521-1-AP (Granzyme B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of NK-92 using 31521-1-AP

    FC experiment of NK-92 using 31521-1-AP

    1x10^6 NK-92 cells were intracellularly stained with 0.25 ug Granzyme B Polyclonal antibody (31521-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Granzyme B Polyclonal antibody

Granzyme B Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 31521-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNK-92 cells, U-937 cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inNK-92 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31521-1-AP targets Granzyme B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0592

Product name: Recombinant Human Granzyme B protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*HIS

Domain: 19-247 aa of BC030195

Sequence: GEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
别名 GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1
计算分子量 247 aa, 28 kDa
观测分子量 33 kDa
GenBank蛋白编号BC030195
基因名称 Granzyme B
Gene ID (NCBI) 3002
RRIDAB_3670018
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP10144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

实验方案

Product Specific Protocols
IHC protocol for Granzyme B antibody 31521-1-APDownload protocol
WB protocol for Granzyme B antibody 31521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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