验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11288-1-AP targets Granzyme A in WB, IHC, IF, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Blocking assay |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
CatNo: Ag1818 Product name: Recombinant human GZMA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-262 aa of BC015739 Sequence: MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLTEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| 别名 | GrA, GZMA, CTL tryptase, CTLA3, Cytotoxic T-lymphocyte proteinase 1 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 27-30 kDa, 50-60 kDa |
| GenBank蛋白编号 | BC015739 |
| 基因名称 | Granzyme A |
| Gene ID (NCBI) | 3001 |
| RRID | AB_2114392 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12544 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Granzyme A antibody 11288-1-AP | Download protocol |
| IHC protocol for Granzyme A antibody 11288-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Exosite-mediated targeting of GSDMB by dimeric granzyme A in lymphocyte pyroptotic killing | ||
Adv Sci (Weinh) PM(10) Impairs CD56(dim) NK Cell Cytotoxicity via FNBP1 Suppression to Exacerbate Rheumatoid Arthritis: Insights from Multimodal Multi-Omics. | ||
Cell Rep Med Spleen-targeted neoantigen mRNA vaccine induces ISG15(+) CD8(+) T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma. | ||
Carbohydr Polym Structural characterization and antitumor activity via immune modulation of an α-glucan from Grifola frondosa. | ||
Cell Death Dis Single-cell transcriptome profiles the heterogeneity of tumor cells and microenvironments for different pathological endometrial cancer and identifies specific sensitive drugs | ||
Int J Biol Sci Rapamycin and Low-dose IL-2 Mediate an Immunosuppressive Microenvironment to Inhibit Benign Prostatic Hyperplasia |
