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验证数据展示

  • WB analysis of human blood using 18147-1-AP (same clone as 18147-1-PBS)

    WB analysis of human blood using 18147-1-AP (same clone as 18147-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 18147-1-AP (GYPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 18147-1-AP (same clone as 18147-1-PBS)

    IF Staining of K-562 using 18147-1-AP (same clone as 18147-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GYPC antibody (18147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

GYPC Polyclonal antibody, PBS Only

GYPC Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 18147-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18147-1-PBS targets GYPC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12825

Product name: Recombinant human GYPC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC106051

Sequence: MWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRMETSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycophorin C (Gerbich blood group)
别名
计算分子量 128 aa, 14 kDa
观测分子量 37 kDa
GenBank蛋白编号BC106051
基因名称 GYPC
Gene ID (NCBI) 2995
RRIDAB_2878508
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04921
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.

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