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验证数据展示

  • WB analysis of L02 using 13150-1-AP

    WB analysis of L02 using 13150-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13150-1-AP (GYG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 13150-1-AP

    IHC staining of mouse liver using 13150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13150-1-AP (GYG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 13150-1-AP

    IHC staining of mouse liver using 13150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13150-1-AP (GYG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GYG2 Polyclonal antibody

GYG2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13150-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

EC:2.4.1.186, glycogenin 2, Glycogenin-2, GN 2, GN2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells, human liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13150-1-AP targets GYG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3765

Product name: Recombinant human GYG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 141-471 aa of BC023152

Sequence: FQPSLHTHKLLLQHAMEHGSFDGADQGLLNSFFRNWSTTDIHKHLPFIYNLSSNTMYTYSPAFKQFGSSAKVVHFLGSMKPWNYKYNPQSGSVLEQGSVSSSQHQAAFLHLWWTVYQNNVLPLYKSVQAGEARASPGHTLCRSDVGGPCADSASGVGEPCENSTPSAGVPCANSPLGSNQPAQGLPEPTQIVDETLSLPEGRRSEDMIACPETETPAVITCDPLSQPSPQPADFTETETILQPANKVESVSSEETFEPSQELPAEALRDPSLQDALEVDLAVSVSQISIEEKVKELSPEEERRKWEEGRIDYMGKDAFARIQEKLDRFLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycogenin 2
别名 EC:2.4.1.186, glycogenin 2, Glycogenin-2, GN 2, GN2
计算分子量 501 aa, 55 kDa
观测分子量 45 kDa, 66-70 kDa
GenBank蛋白编号BC023152
基因名称 GYG2
Gene ID (NCBI) 8908
RRIDAB_2116111
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15488
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GYG2 belongs to the glycogenin family. Glycogenin is a self-glucosylating protein involved in the initiation phase of glycogen biosynthesis. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, including the predominant band around 66 kDa and a light band around 45 kDa.

实验方案

Product Specific Protocols
IHC protocol for GYG2 antibody 13150-1-APDownload protocol
WB protocol for GYG2 antibody 13150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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