验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U-251 cells |
Planning an IHC experiment? We recommend our IHCeasy GYG1 Ready-To-Use IHC Kit. GYG1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86056-1-RR targets GYG1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GYG1 fusion protein Ag3583 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glycogenin 1 |
| 别名 | EC:2.4.1.186, glycogenin 1, Glycogenin-1, GN1, GN-1 |
| 计算分子量 | 350 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC031096 |
| 基因名称 | GYG1 |
| Gene ID (NCBI) | 2992 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46976 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The GYG1 gene encodes glycogenin-1, a core protein in muscle glycogen particles. GYG1 is a glycosyltransferase that forms a short glucose polymer of approximately 10 glucose residues by autoglucosylation (PMID: 25272951 ). Cardiomyopathy and muscle weakness associated with muscle glycogen depletion can also be caused by impaired priming of glycogen synthesis due to inactivation of muscle glycogenin (glycogenin-1) (PMID: 20357282).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GYG1 antibody 86056-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |