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验证数据展示

  • WB analysis using 19902-1-AP

    WB analysis using 19902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19902-1-AP (GULP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 19902-1-AP

    IHC staining of human stomach cancer using 19902-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19902-1-AP (GULP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GULP1 Polyclonal antibody

GULP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19902-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PTB domain adapter protein CED-6, Protein GULP, GULP, Cell death protein 6 homolog, CED6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19902-1-AP targets GULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag13829

Product name: Recombinant human GULP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-133 aa of BC001103

Sequence: MNRAFSRKKDKTWMHTPEALSKHFIPYNAKFLGSTEVEQPKGTEVVRDAVRKLKFARHIKKSEGQKIPKVELQISIYGVKILEPKTKEVQHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCYVFDSEKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GULP, engulfment adaptor PTB domain containing 1
别名 PTB domain adapter protein CED-6, Protein GULP, GULP, Cell death protein 6 homolog, CED6
计算分子量 167 aa, 19 kDa
观测分子量34 kDa
GenBank蛋白编号BC001103
基因名称 GULP1
Gene ID (NCBI) 51454
RRIDAB_10916254
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GULP1 antibody 19902-1-APDownload protocol
IHC protocol for GULP1 antibody 19902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer

Authors - Dong Yan
View Article
mouseWB

Aging (Albany NY)

GATA2 downregulation contributes to pro-inflammatory phenotype and defective phagocytosis of pulmonary macrophages in chronic obstructive pulmonary disease

Authors - Shaoran Shen
View Article