验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human thyroid cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy GUK1 Ready-To-Use IHC Kit. GUK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67047-1-Ig targets GUK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag28805 Product name: Recombinant human GUK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-197 aa of BC009914 Sequence: MSGPRPVVLSGPSGAGKSTLLKRLLQEHSGIFGFSVSHTTRNPRPGEENGKDYYFVTREVMQRDIAAGDFIEHAEFSGNLYGTSKVAVQAVQAMNRICVLDVDLQGVRNIKATDLRPIYISVQPPSLHVLEQRLRQRNTETEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | guanylate kinase 1 |
| 别名 | EC:2.7.4.8, GMK, GMP kinase, GMPK, Guanylate kinase |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22-27 kDa |
| GenBank蛋白编号 | BC009914 |
| 基因名称 | GUK1 |
| Gene ID (NCBI) | 2987 |
| RRID | AB_2882360 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q16774 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GUK1 antibody 67047-1-Ig | Download protocol |
| IHC protocol for GUK1 antibody 67047-1-Ig | Download protocol |
| WB protocol for GUK1 antibody 67047-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
