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验证数据展示

  • WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

    WB analysis of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

    WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • WB analysis using 17489-1-AP (same clone as 17489-1-PBS)

    WB analysis using 17489-1-AP (same clone as 17489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

    IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

    IHC staining of human oesophagus using 17489-1-AP (same clone as 17489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

    IF Staining of HepG2 using 17489-1-AP (same clone as 17489-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 17489-1-AP (same clone as 17489-1-PBS)

    IP experiment of HeLa using 17489-1-AP (same clone as 17489-1-PBS)

    IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 17489-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GUF1 Polyclonal antibody, PBS Only

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17489-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17489-1-PBS targets GUF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11579

Product name: Recombinant human GUF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-669 aa of BC036768

Sequence: VGYLIAGMKDVTEAQIGDTLCLHKQPVEPLPGFKSAKPMVFAGMYPLDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHMEVFNQRLEQEYNASVILTTPTVPYKAVLSSSKLIKEHREKEITIINPAQFPDKSKVTEYLEPVVLGTIITPDEYTGKIMMLCEARRAVQKNMIFIDQNRVMLKYLFPLNEIVVDFYDSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNTVEELVTVVHKDKAHSIGKAICERLKDSLPRQLFEIAIQAAIGSKIIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKLRKIGNVEVPKDAFIKVLKTQSSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GUF1 GTPase homolog (S. cerevisiae)
别名
计算分子量 669 aa, 74 kDa
观测分子量 70 kDa
GenBank蛋白编号BC036768
基因名称 GUF1
Gene ID (NCBI) 60558
RRIDAB_2115960
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N442
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

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