验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
25252-1-AP targets GUCY2F in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | GUCY2F fusion protein Ag19266 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate cyclase 2F, retinal |
| 别名 | CYGF, GC F, guanylate cyclase 2F, retinal, Guanylate cyclase F, GUC2DL, GUC2F, GUCY2F, RETGC 2, RETGC2, Retinal guanylyl cyclase 2, ROS GC2 |
| 计算分子量 | 1108 aa, 125 kDa |
| 观测分子量 | 125 kDa |
| GenBank蛋白编号 | BC156674 |
| 基因名称 | GUCY2F |
| Gene ID (NCBI) | 2986 |
| RRID | AB_2879989 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51841 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GUCY2F(Guanylate cyclase 2F, retinal) is also named as GUC2F, RETGC2 and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. GUCY2F may play a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. By in situ hybridization, mRNA encoding GUCY2F is found only in the outer nuclear layer and inner segments of photoreceptor cells. By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2(GUCY2F) can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments.(PMID:7777544).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GUCY2F antibody 25252-1-AP | Download protocol |
| IHC protocol for GUCY2F antibody 25252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
Transl Vis Sci Technol Construction and Identification of a Novel Mice Model of Microphthalmia |