验证数据展示
经过测试的应用
| Positive IHC detected in | rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21531-1-AP targets GUCA1C in IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag16157 Product name: Recombinant human GUCA1C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC103993 Sequence: MGNGKSIAGDQKAVPTQETHVWYRTFMMEYPSGLQTLHEFKTLLGLQGLNQKANKHIDQVYNTFDTNKDGFIDFLEFIAAVNLIVQEKMEQKLKWYFKLYDADGNGSIDKNELLDMFMAVQALNGQQTLSPEEFINLVFHKIDINNDGELTLEEFINGMAKDQDLLEIVYKSFDFSNVLRVICNGKQPDMETDSSKSPDKAGLGKVKMK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate cyclase activator 1C |
| 别名 | GCAP 3, GCAP3, guanylate cyclase activator 1C, Guanylyl cyclase-activating protein 3 |
| 计算分子量 | 209 aa, 24 kDa |
| GenBank蛋白编号 | BC103993 |
| 基因名称 | GUCA1C |
| Gene ID (NCBI) | 9626 |
| RRID | AB_3742042 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95843 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The guanylate cyclase-activating proteins (GCAPs), Ca2+-binding proteins of the calmodulin gene superfamily, function as regulators of photoreceptor guanylate cyclases (PMID: 15336959). Three different GCAPs (GCAP1, GCAP2, and GCAP3) are identified in vertebrate retina (PMID: 12596930). The cone-specific guanylate cyclase-activating protein 3 (GCAP3) has been shown to regulate the enzymatic activity of membrane-bound guanylate cyclases (GCs) in bovine and teleost fish photoreceptors, to an extent comparable to that of the paralog protein GCAP1 (PMID: 35328663).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GUCA1C antibody 21531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
