验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse liver tissue, K-562 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human stomach cancer tissue, human placenta tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21319-1-AP targets GTSE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag13497 Product name: Recombinant human GTSE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 416-720 aa of BC006325 Sequence: CAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSARRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G-2 and S-phase expressed 1 |
| 别名 | G 2 and S phase expressed 1, G2 and S phase-expressed protein 1, GTSE 1, GTSE-1, Protein B99 homolog |
| 计算分子量 | 77 kDa |
| 观测分子量 | 66-77 kDa, 100 kDa |
| GenBank蛋白编号 | BC006325 |
| 基因名称 | GTSE1 |
| Gene ID (NCBI) | 51512 |
| RRID | AB_10733644 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GTSE1 antibody 21319-1-AP | Download protocol |
| WB protocol for GTSE1 antibody 21319-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner. | ||
Mol Ther GTSE1-driven ZEB1 Stabilization Promotes Pulmonary Fibrosis Through the Epithelial-to-mesenchymal Transition
| ||
Proc Natl Acad Sci U S A The physiological relevance of downstream effectors of p53 activity. | ||
Mod Pathol Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets. | ||
Mol Ther Nucleic Acids lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation. |
