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- KD/KO Validated
GTPBP4 Polyclonal antibody
GTPBP4 Polyclonal Antibody for IF/ICC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, human kidney tissue, HepG2 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13897-1-AP targets GTPBP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IP, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, Drosophila |
| Immunogen | GTPBP4 fusion protein Ag4859 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | GTP binding protein 4 |
| Synonyms | CRFG, GTP binding protein 4, GTP binding protein NGB, GTPBP4, NGB, NOG1 |
| Calculated Molecular Weight | 634 aa, 74 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC038975 |
| Gene Symbol | GTPBP4 |
| Gene ID (NCBI) | 23560 |
| RRID | AB_2279486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| IF protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| IP protocol for GTPBP4 antibody 13897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. | ||
Proc Natl Acad Sci U S A A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.
| ||
Biochem Biophys Res Commun Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis.
| ||
Biomed Res Int Determining the Clinical Value and Critical Pathway of GTPBP4 in Lung Adenocarcinoma Using a Bioinformatics Strategy: A Study Based on Datasets from The Cancer Genome Atlas.
| ||
Cell Rep UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer |
