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验证数据展示

  • WB analysis of human kidney using 11557-1-AP

    WB analysis of human kidney using 11557-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 11557-1-AP

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 11557-1-AP

    IHC staining of human pancreas using 11557-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of mouse spleen using 11557-1-AP

    IP experiment of mouse spleen using 11557-1-AP

    IP result of anti-GTPBP2 (IP:11557-1-AP, 4ug; Detection:11557-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

GTPBP2 Polyclonal antibody

GTPBP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11557-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

GTP binding protein 2, GTPBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, human spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11557-1-AP targets GTPBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2113

Product name: Recombinant human GTPBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-532 aa of BC028347

Sequence: SKMITFIDLAGHHKYLHTTIFGLTSYCPDCALLLVSANTGIAGTTREHLGLALALKVPFFIVVSKIDLCAKTTVERTVRQLERVLKQPGCHKVPMLVTSEDDAVTAAQQFAQSPNVTPIFTLSSVSGESLDLLKVFLNILPPLTNSKEQEELMQQLTEFQVDEIYTVPEVGTVVGGTLSSGICREGDQLVVGPTDDGCFLELRVCSIQRNRSACRVLRAGQAATLALGDFDRALLRKGMVMLSPEMNPTICSVFEAEIVLLFHATTFRRGFQVTVHVGNVRQTAVVEKIHAKDKLRTGEKAVVRFRFLKHPEYLKVGAKLLFREGVTKGIGHVTDVQAITAGEAQANMGF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 2
别名 GTP binding protein 2, GTPBP2
计算分子量 602 aa, 66 kDa
观测分子量 60 kDa
GenBank蛋白编号BC028347
基因名称 GTPBP2
Gene ID (NCBI) 54676
RRIDAB_2115381
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BX10
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP2 antibody 11557-1-APDownload protocol
IHC protocol for GTPBP2 antibody 11557-1-APDownload protocol
IP protocol for GTPBP2 antibody 11557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols