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验证数据展示

  • WB analysis of mouse skeletal muscle using 16374-1-AP

    WB analysis of mouse skeletal muscle using 16374-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16374-1-AP (GTPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 16374-1-AP

    IP experiment of mouse skeletal muscle using 16374-1-AP

    IP result of anti-GTPBP1 (IP:16374-1-AP, 4ug; Detection:16374-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

GTPBP1 Polyclonal antibody

GTPBP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16374-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

G protein 1, GP 1, GP1, GTP binding protein 1, GTPBP1, HSPC018

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, Jurkat cells
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16374-1-AP targets GTPBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9332

Product name: Recombinant human GTPBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 469-666 aa of BC014075

Sequence: SIRKGMVMVSPRLNPQASWEFEAEILVLHHPTTISPRYQAMVHCGSIRQTATILSMDKDCLRTGDKATVHFRFIKTPEYLHIDQRLVFREGRTKAVGTITKLLQTTNNSPMNSKPQQIKMQSTKKGPLTKRDEGGPSGGPAVGAPPPGDEASSVGAGQPAASSNLQPQPKPSSGGRRRGGQRHKVKSQGACVTPASGC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 1
别名 G protein 1, GP 1, GP1, GTP binding protein 1, GTPBP1, HSPC018
计算分子量 660 aa, 71 kDa
观测分子量 72 kDa
GenBank蛋白编号BC014075
基因名称 GTPBP1
Gene ID (NCBI) 9567
RRIDAB_2115267
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00178
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTPBP1 antibody 16374-1-APDownload protocol
IP protocol for GTPBP1 antibody 16374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols