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验证数据展示

  • WB analysis using 19905-1-AP

    WB analysis using 19905-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19905-1-AP (GTL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 19905-1-AP

    IHC staining of mouse testis using 19905-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19905-1-AP (GTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTL3 antibody included
  • IHC staining of mouse testis using 19905-1-AP

    IHC staining of mouse testis using 19905-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19905-1-AP (GTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTL3 antibody included

GTL3 Polyclonal antibody

GTL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19905-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C16orf80, Basal body up-regulated protein 22, BUG22, CFAP20, Cilia- and flagella-associated protein 20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTL3 Ready-To-Use IHC Kit. GTL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19905-1-AP targets GTL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GTL3 fusion protein Ag13881 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 80
别名 C16orf80, Basal body up-regulated protein 22, BUG22, CFAP20, Cilia- and flagella-associated protein 20
计算分子量 193 aa, 23 kDa
观测分子量22 kDa
GenBank蛋白编号BC005152
基因名称 GTL3
Gene ID (NCBI) 29105
RRIDAB_3669317
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6A4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTL3 (Cilia- and flagella-associated protein 20, also named CFAP20 ) may play a role in axonemal structure organization and motility.

实验方案

Product Specific Protocols
WB protocol for GTL3 antibody 19905-1-APDownload protocol
IHC protocol for GTL3 antibody 19905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols