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验证数据展示

  • WB analysis of HeLa using 26382-1-AP

    WB analysis of HeLa using 26382-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26382-1-AP (GTF3C6 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26382-1-AP

    IP experiment of HepG2 using 26382-1-AP

    IP result of anti-GTF3C6 (IP:26382-1-AP, 4ug; Detection:26382-1-AP 1:2000) with HepG2 cells lysate 3600ug.

  • IHC staining of human urothelial carcinoma using 26382-1-AP

    IHC staining of human urothelial carcinoma using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included
  • IHC staining of mouse lung using 26382-1-AP

    IHC staining of mouse lung using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included
  • IHC staining of human lung cancer using 26382-1-AP

    IHC staining of human lung cancer using 26382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26382-1-AP (GTF3C6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF3C6 antibody included

GTF3C6 Polyclonal antibody

GTF3C6 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

TFIIIC35, NPD020, CDA020, C6orf51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human lung cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF3C6 Ready-To-Use IHC Kit. GTF3C6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26382-1-AP targets GTF3C6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 GTF3C6 fusion protein Ag23895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIIC, polypeptide 6, alpha 35kDa
别名 TFIIIC35, NPD020, CDA020, C6orf51
观测分子量 35-38 kDa
GenBank蛋白编号BC011593
基因名称 GTF3C6
Gene ID (NCBI) 112495
RRIDAB_2880497
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969F1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF3C6 antibody 26382-1-APDownload protocol
IHC protocol for GTF3C6 antibody 26382-1-APDownload protocol
IP protocol for GTF3C6 antibody 26382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols