验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32916-1-AP targets GTF3C3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38352 Product name: Recombinant human GTF3C3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-373 aa of NM_012086.4 Sequence: MVLNRETKKMMKEKRPRSKLPRALRGLMGEANIRFARGEREEAILMCMEIIRQAPLAYEPFSTLAMIYEDQGDMEKSLQFELIAAHLNPSDTEEWVRLAEMSLEQDNIKQAIFCYTKALKYEPTNVRYLWERSSLYEQMGDHKMAMDGYRRILNLLSPSDGERFMQLARDMAKSYYEANDVTSAINIIDEAFSKHQGLVSMEDVNIAAELYISNKQYDKALEIITDFSGIVLEKKTSEEGTSEENKAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIIC, polypeptide 3, 102kDa |
| 别名 | General transcription factor 3C polypeptide 3, TF3C-gamma, TFIIIC 102 kDa subunit, TFIIIC102, Transcription factor IIIC 102 kDa subunit |
| 计算分子量 | 101kDa，886aa |
| 观测分子量 | 101 kDa |
| GenBank蛋白编号 | NM_012086.4 |
| 基因名称 | GTF3C3 |
| Gene ID (NCBI) | 9330 |
| RRID | AB_3742786 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y5Q9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTF3C3 (General Transcription Factor 3C Subunit 3) is a key component of the RNA polymerase III (Pol III) transcription machinery, which is essential for the synthesis of small non-coding RNAs, including tRNAs, 5S rRNA, and other small regulatory RNAs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF3C3 antibody 32916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
