验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33192-1-AP targets GTF3A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35931 Product name: Recombinant human GTF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-365 aa of NM_002097 Sequence: KTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIIA |
| 别名 | TFIIIA, Transcription factor IIIA |
| 计算分子量 | 41kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | NM_002097 |
| 基因名称 | GTF3A |
| Gene ID (NCBI) | 2971 |
| RRID | AB_3742887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q92664 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTF3A encodes general transcription factor IIIA (TFIIIA) whose role in transcriptional regulation of 5S ribosomal DNA genes (encoding 5S ribosomal RNA (rRNA)) has been well characterized. TFIIIA comprises nine zinc finger (ZF) domains, each containing two invariant pairs of cysteines and histidines coordinating one Zn2+ ion (PMID: 36399538).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF3A antibody 33192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
