验证数据展示
经过测试的应用
| Positive IHC detected in | mouse kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29112-1-AP targets GTF2H5 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag30758 Product name: Recombinant human GTF2H5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-42 aa of BC060317 Sequence: MVNVLKGVLIECDPAMKQFLLYLDESNALGKKFIIQDIDDTH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIH, polypeptide 5 |
| 别名 | bA120J8.2, C6orf175, TFB5, TFB5 ortholog, TFIIH basal transcription factor complex TTD-A subunit |
| 计算分子量 | 8 kDa |
| GenBank蛋白编号 | BC060317 |
| 基因名称 | GTF2H5 |
| Gene ID (NCBI) | 404672 |
| RRID | AB_3742304 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZYL4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTF2H5 (General Transcription Factor IIH Subunit 5) is a protein encoded by the GTF2H5 gene in humans. It is also known by several other names, including TTDA, TFB5, and TFIIH subunit p8. This protein is a critical component of the TFIIH complex, which is involved in both gene transcription and DNA repair.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GTF2H5 antibody 29112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
