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GTF2H2C Polyclonal antibody

GTF2H2C Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17959-1-AP

Print datasheet

Synonyms

GTF2H2C, GTF2H2D

验证数据展示

  • WB analysis using 17959-1-AP

    WB analysis using 17959-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 17959-1-AP

    IP experiment of HepG2 using 17959-1-AP

    IP result of anti-GTF2H2C (IP:17959-1-AP, 4ug; Detection:17959-1-AP 1:500) with HepG2 cells lysate 2240 ug.

  • WB analysis of HepG2 using 17959-1-AP

    WB analysis of HepG2 using 17959-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inL02 cells, HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17959-1-AP targets GTF2H2C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen GTF2H2C fusion protein Ag12498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIH, polypeptide 2C
Synonyms GTF2H2C, GTF2H2D
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC105980
Gene Symbol GTF2H2C
Gene ID (NCBI) 728340
RRIDAB_2114793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2H2C antibody 17959-1-APDownload protocol
IP protocol for GTF2H2C antibody 17959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols