验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16005-1-AP targets GTF2H2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8653 Product name: Recombinant human GTF2H2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-165 aa of BC005345 Sequence: MDEEPERTKRWEGGYERTWEILKEDESGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRTMEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKKAVDMTCHGEPSLYNSLSIAMQTLKLVLYIMYN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIH, polypeptide 2, 44kDa |
| 别名 | BTF2, BTF2 p44, BTF2P44, T BTF2P44, TFIIH |
| 计算分子量 | 395aa,44 kDa; 165aa,19 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC005345 |
| 基因名称 | GTF2H2 |
| Gene ID (NCBI) | 2966 |
| RRID | AB_2279415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13888 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcription factor IIH(TFIIH) is associated with the RNA polymerase II transcription complex, which is involved in transcription and transcription-mediated DNA repair. GTF2H2(GENERAL TRANSCRIPTION FACTOR IIH, POLYPEPTIDE 2) is one of the six subnuits forming the core-TFIIH basal transcriptional factor. TFIIH plays a role in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N terminus of GTF2H2 interacts with and regulates XPD, and an intact C terminus is required for a successful escape of RNAPol II from the promoter. This is a rabbit polyclonal antibody producted by part of N-terminal GTF2H2 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF2H2 antibody 16005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
