验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, mouse brain tissue, HepG2 cells, HL-60 cells, rat brain tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10173-1-AP targets GTF2H1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | GTF2H1 fusion protein Ag0229 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | general transcription factor IIH, polypeptide 1, 62kDa |
别名 | BTF2, BTF2 p62, GTF2H1, TFB1, TFIIH |
计算分子量 | 62 kDa |
观测分子量 | 62 kDa |
GenBank蛋白编号 | BC004452 |
基因名称 | GTF2H1 |
Gene ID (NCBI) | 2965 |
RRID | AB_2295009 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P32780 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFIIH is a multiprotein complex that has a central role in the RNA pol II mediated transcription, in DNA repair and in the control of the cell cycle. TFIIH consists of nine subunits: cyclin-dependent kinase 7 (Cdk7), cyclin H and MAT1 (forming the Cdk-activating-kinase or CAK complex), the two helicases Xpb/Hay and Xpd, and p34, p44, p52 and p62. As the kinase subunit of TFIIH, Cdk7 participates in basal transcription by phosphorylating the carboxy-terminal domain of the largest subunit of RNA polymerase II. Mutations in some components of TFIIH are associated with three hereditary human syndromes: xeroderma pigmentosum (XP), Cockayne syndrome (CS) and trichothiodystrophy (TTD). The p62 protein (TFIIH1) is a structural component of the TFIIH core and no syndromes have been identified up to date by mutations in this human gene.
实验方案
Product Specific Protocols | |
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WB protocol for GTF2H1 antibody 10173-1-AP | Download protocol |
IHC protocol for GTF2H1 antibody 10173-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |