GTF2H1 Polyclonal antibody

GTF2H1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10173-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

BTF2, BTF2 p62, GTF2H1, TFB1, TFIIH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HepG2 cells, HL-60 cells, rat brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF2H1 Ready-To-Use IHC Kit. GTF2H1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10173-1-AP targets GTF2H1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GTF2H1 fusion protein Ag0229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIH, polypeptide 1, 62kDa
别名 BTF2, BTF2 p62, GTF2H1, TFB1, TFIIH
计算分子量 62 kDa
观测分子量 62 kDa
GenBank蛋白编号BC004452
基因名称 GTF2H1
Gene ID (NCBI) 2965
RRIDAB_2295009
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32780
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFIIH is a multiprotein complex that has a central role in the RNA pol II mediated transcription, in DNA repair and in the control of the cell cycle. TFIIH consists of nine subunits: cyclin-dependent kinase 7 (Cdk7), cyclin H and MAT1 (forming the Cdk-activating-kinase or CAK complex), the two helicases Xpb/Hay and Xpd, and p34, p44, p52 and p62. As the kinase subunit of TFIIH, Cdk7 participates in basal transcription by phosphorylating the carboxy-terminal domain of the largest subunit of RNA polymerase II. Mutations in some components of TFIIH are associated with three hereditary human syndromes: xeroderma pigmentosum (XP), Cockayne syndrome (CS) and trichothiodystrophy (TTD). The p62 protein (TFIIH1) is a structural component of the TFIIH core and no syndromes have been identified up to date by mutations in this human gene.

实验方案

Product Specific Protocols
WB protocol for GTF2H1 antibody 10173-1-APDownload protocol
IHC protocol for GTF2H1 antibody 10173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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