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验证数据展示

  • WB analysis of HEK-293 using 24405-1-AP

    WB analysis of HEK-293 using 24405-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24405-1-AP (GTF2E1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 24405-1-AP

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of rat kidney using 24405-1-AP

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of mouse kidney using 24405-1-AP

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of mouse kidney using 24405-1-AP

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human stomach cancer using 24405-1-AP

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human stomach cancer using 24405-1-AP

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human urothelial carcinoma using 24405-1-AP

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included
  • IHC staining of human urothelial carcinoma using 24405-1-AP

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GTF2E1 antibody included

GTF2E1 Polyclonal antibody

GTF2E1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24405-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat kidney tissue, mouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF2E1 Ready-To-Use IHC Kit. GTF2E1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24405-1-AP targets GTF2E1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原 GTF2E1 fusion protein Ag19794 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIE, polypeptide 1, alpha 56kDa
别名 FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha
计算分子量 439 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC156997
基因名称 GTF2E1
Gene ID (NCBI) 2960
RRIDAB_2879526
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29083
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GTF2E1 antibody 24405-1-APDownload protocol
IHC protocol for GTF2E1 antibody 24405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

bioRxiv

Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends

Authors - Olivia Luyties
View Article