验证数据展示
产品信息
87643-2-PBS targets GTF2B as part of a matched antibody pair:
MP03104-1: 87643-2-PBS capture and 87643-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Cytometric bead array, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9547 Product name: Recombinant human GTF2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC020597 Sequence: MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | general transcription factor IIB |
| 别名 | EC:2.3.1.48, S300 II, S300-II, TF2B, TFIIB |
| 计算分子量 | 316 aa, 35 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC020597 |
| 基因名称 | GTF2B |
| Gene ID (NCBI) | 2959 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00403 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GTF2B encodes transcription factor IIB (TFIIB), one of the ubiquitous general transcription factors essential for transcription initiation by RNA polymerase II (Pol II). Localizing to the nucleus, TFIIB forms a complex with transcription factors IID and IIA and serves as a critical bridge between TFIID-the factor that initially recognizes the promoter-and the Pol II machinery, thereby enabling pre-initiation complex (PIC) assembly and Pol II recruitment to promoter DNA. Beyond this core architectural role, TFIIB is subject to regulatory acetylation and autoacetylation, and emerging evidence links its dysregulation to pathological states, including potential contributions to proliferation in hepatocellular carcinoma and tumor phenotypes in growth hormone-secreting pituitary adenomas.
