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验证数据展示

  • WB analysis of mouse testis using 10540-1-AP

    WB analysis of mouse testis using 10540-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10540-1-AP (GTF2A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10540-1-AP

    IHC staining of mouse liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GTF2A2 Polyclonal antibody

GTF2A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells
Positive IHC detected inmouse liver tissue, mouse heart tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10540-1-AP targets GTF2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0825

Product name: Recombinant human GTF2A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-109 aa of BC001919

Sequence: MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 general transcription factor IIA, 2, 12kDa
别名 TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2
计算分子量 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC001919
基因名称 GTF2A2
Gene ID (NCBI) 2958
RRIDAB_2114351
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52657
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region [PMID:20480367]. TFIIA in a complex with TBP mediates transcriptional activity [PMID: 11030333]. GTF2A2 is one subunit of TFIIA.

实验方案

Product Specific Protocols
WB protocol for GTF2A2 antibody 10540-1-APDownload protocol
IHC protocol for GTF2A2 antibody 10540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols