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验证数据展示

  • WB analysis using 66578-1-Ig

    WB analysis using 66578-1-Ig

    Various tissue were subjected to SDS PAGE followed by western blot with 66578-1-Ig (GTDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66578-1-Ig

    IHC staining of human testis using 66578-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 66578-1-Ig

    IHC staining of human testis using 66578-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 66578-1-Ig

    IF Staining of U2OS using 66578-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 66578-1-Ig (GTDC1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

GTDC1 Monoclonal antibody

GTDC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66578-1-Ig

产品说明书

CloneNo. 1C4C10

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

GTDC1, mat Xa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, pig brain tissue, pig cerebellum tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66578-1-Ig targets GTDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig
免疫原

CatNo: Ag9934

Product name: Recombinant human GTDC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-292 aa of BC061699

Sequence: MSILIIEAFYGGSHKQLVDLLQEELGDCVVYTLPAKKWHWRARTSALYFSQTIPISEHYRTLFASSVLNLTELAALRPDLGKLKKILYFHENQLIYPVKKCQERDFQYGYNQILSCLVADVVVFNSVFNMESFLTSMGKFMKLIPDHRPKDLESIIRPKCQVIYFPIRFPDVSRFMPKHKTTHLKKMLGLKGNGGAVLSMALPFQPEQRDSEDLLKNFNSECDTHCGLDTARQEYLGNSLRQESDLKKSTSSDNSSSHHGENKQNLTVDPCDILGGVDNQQRLLHIVWPHRW

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycosyltransferase-like domain containing 1
别名 GTDC1, mat Xa
计算分子量 292 aa, 34 kDa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC061699
基因名称 GTDC1
Gene ID (NCBI) 79712
RRIDAB_2881938
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ4AE62
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for GTDC1 antibody 66578-1-IgDownload protocol
IHC protocol for GTDC1 antibody 66578-1-IgDownload protocol
WB protocol for GTDC1 antibody 66578-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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