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验证数据展示

  • IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTT1 antibody included
  • WB analysis using 67645-1-Ig (same clone as 67645-1-PBS)

    WB analysis using 67645-1-Ig (same clone as 67645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTT1 antibody included
  • IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

GSTT1 Monoclonal antibody, PBS Only

GSTT1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67645-1-PBS

产品说明书

CloneNo. 1A4C11

宿主/亚型

Mouse / IgG2b

种属反应性

Human, pig, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

Glutathione transferase T1 1, GST class theta 1, GSTT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSTT1 Ready-To-Use IHC Kit. GSTT1 primary antibody included.

产品信息

67645-1-PBS targets GSTT1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, pig, mouse
免疫原

CatNo: Ag8573

Product name: Recombinant human GSTT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of BC007065

Sequence: MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDACAQVNPLKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLAWQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutathione S-transferase theta 1
别名 Glutathione transferase T1 1, GST class theta 1, GSTT1
计算分子量 240 aa, 27 kDa
观测分子量 28 kDa, 30 kDa
GenBank蛋白编号BC007065
基因名称 GSTT1
Gene ID (NCBI) 2952
RRIDAB_2882845
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30711
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.