验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00121 targets GSTP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase pi 1 |
| 别名 | EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3, GST3/GST pi |
| 计算分子量 | 210 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC010915 |
| 基因名称 | GSTP1 |
| Gene ID (NCBI) | 2950 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P09211 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glutathione S-transferase Pi (GSTP1) is anisozyme encoded by the GST pi gene that plays an importantregulatory role in detoxification, anti‑oxidative damage, and the occurrence of various diseases (PMID: 14755684). GSTP1 conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PMID:9084911). GSTP1 methylation is frequently associated with tumor development or poor prognosis in a wide range of tumors such as neuroblastoma, hepatocellular carcinoma, endometrial, breast, and prostate cancers (PCa) (PMID: 27594734).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTP1 antibody RMX00121 | Download protocol |
| IHC protocol for GSTP1 antibody RMX00121 | Download protocol |
| WB protocol for GSTP1 antibody RMX00121 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
