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验证数据展示

  • WB analysis using 60150-2-Ig (same clone as 60150-2-PBS)

    WB analysis using 60150-2-Ig (same clone as 60150-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 60150-2-Ig (same clone as 60150-2-PBS)

    WB analysis of human heart using 60150-2-Ig (same clone as 60150-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTO1 antibody included
  • IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTO1 antibody included
  • IF Staining of PC-3 using 60150-2-Ig (same clone as 60150-2-PBS)

    IF Staining of PC-3 using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (60150-2-Ig, Clone: 5E1E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

GSTO1 Monoclonal antibody, PBS Only

GSTO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60150-2-PBS

产品说明书

CloneNo. 5E1E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:1.20.4.2, EC:1.8.5.1, EC:2.5.1.18, glutathione S transferase omega 1, Glutathione S-transferase omega 1-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSTO1 Ready-To-Use IHC Kit. GSTO1 primary antibody included.

产品信息

60150-2-PBS targets GSTO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7199

Product name: Recombinant human GSTO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC000127

Sequence: MSGESARSLGKGSAPPGPVPEGSIRIYSMRFCPFAERTRLVLKAKGIRHEVININLKNKPEWFFKKNPFGLVPVLENSQGQLIYESAITCEYLDEAYPGKKLLPDDPYEKACQKMILELFSKVPSLVGSFIRSQNKEDYAGLKEEFRKEFTKLEEVLTNKKTTFFGGNSISMIDYLIWPWFERLEAMKLNECVDHTPKLKLWMAAMKEDPTVSALLTSEKDWQGFLELYLQNSPEACDYGL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutathione S-transferase omega 1
别名 EC:1.20.4.2, EC:1.8.5.1, EC:2.5.1.18, glutathione S transferase omega 1, Glutathione S-transferase omega 1-1
计算分子量 28 kDa
观测分子量 27-30 kDa
GenBank蛋白编号BC000127
基因名称 GSTO1
Gene ID (NCBI) 9446
RRIDAB_10644447
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78417
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells.

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