验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat skeletal muscle tissue, mouse testis |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14502-1-AP targets GSTM5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5958 Product name: Recombinant human GSTM5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC058881 Sequence: MPMTLGYWDIRGLAHAIRLLLEYTDSSYVEKKYTLGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGETEEEKIRVDILENQVMDNHMELVRLCYDPDFEKLKPKYLEELPEKLKLYSEFLGKRPWFAGDKITFVDFLAYDVLDMKRIFEPKCLDAFLNLKDFISRFEGLKKISAYMKSSQFLRGLLFGKSATWNSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase mu 5 |
| 别名 | GSTM 5, GST class-mu 5, GST class mu 5, Glutathione S-transferase Mu 5, glutathione S transferase mu 5 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC058881 |
| 基因名称 | GSTM5 |
| Gene ID (NCBI) | 2949 |
| RRID | AB_2263888 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46439 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSTM5 belongs to the GST superfamily that metabolizes a broad range of xenobiotics and carcinogens. GSTM5 is expressed in brain, testis, lung and a lesser extent in heart.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTM5 antibody 14502-1-AP | Download protocol |
| IHC protocol for GSTM5 antibody 14502-1-AP | Download protocol |
| IP protocol for GSTM5 antibody 14502-1-AP | Download protocol |
| WB protocol for GSTM5 antibody 14502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteomics Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity. | ||
J Neurol Sci Proteomic analysis of the hippocampus in naïve and ischemic-preconditioned rat. | ||
Andrology Thiol changes during epididymal maturation: a link to flagellar angulation in mouse spermatozoa? | ||
Cell Death Discov IGF2BP2 regulates the inflammation of fibroblast-like synoviocytes via GSTM5 in rheumatoid arthritis
| ||
Drug Des Devel Ther The Mechanism of Xuanyu Tongjing Decoction Regulating NOD/NFκB Pathway to Inhibit Ectopic Tissue Inflammation to Reduce Ovarian Damage in Rats with Ovarian Endometriosis |
