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GSTM3 Monoclonal antibody

GSTM3 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1E2B8

Cat No : 67634-1-Ig

Print datasheet

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

验证数据展示

  • WB analysis of HepG2 using 67634-1-Ig

    WB analysis of HepG2 using 67634-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67634-1-Ig

    IF Staining of A431 using 67634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GSTM3 antibody (67634-1-Ig, Clone: 1E2B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, pig brain tissue
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy GSTM3 Ready-To-Use IHC Kit. GSTM3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67634-1-Ig targets GSTM3 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsIF, CoIP
Tested Reactivity Human, pig
Cited Reactivityhuman
Immunogen GSTM3 fusion protein Ag7494 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase mu 3 (brain)
Synonyms Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000088
Gene Symbol GSTM3
Gene ID (NCBI) 2947
RRIDAB_2882835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21266
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

实验方案

Product Specific Protocols
WB protocol for GSTM3 antibody 67634-1-IgDownload protocol
IF protocol for GSTM3 antibody 67634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,CoIP

Reprod Biol Endocrinol

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Authors - Gelin Huang
View Article