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验证数据展示

  • WB analysis of HepG2 using 67634-1-Ig

    WB analysis of HepG2 using 67634-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67634-1-Ig

    IF Staining of A431 using 67634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GSTM3 antibody (67634-1-Ig, Clone: 1E2B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

GSTM3 Monoclonal antibody

GSTM3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67634-1-Ig

产品说明书

CloneNo. 1E2B8
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, ELISA and More (2)

别名

1E2B8, EC:2.5.1.18, Glutathione S transferase Mu 3, Glutathione S-transferase Mu 3, GST class mu 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, pig brain tissue
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy GSTM3 Ready-To-Use IHC Kit. GSTM3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67634-1-Ig targets GSTM3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF, CoIP
经测试反应性 human, pig
文献引用反应性human
免疫原

CatNo: Ag7494

Product name: Recombinant human GSTM3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC000088

Sequence: MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutathione S-transferase mu 3 (brain)
别名 1E2B8, EC:2.5.1.18, Glutathione S transferase Mu 3, Glutathione S-transferase Mu 3, GST class mu 3
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC000088
基因名称 GSTM3
Gene ID (NCBI) 2947
RRIDAB_2882835
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21266
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

实验方案

Product Specific Protocols
IF protocol for GSTM3 antibody 67634-1-IgDownload protocol
WB protocol for GSTM3 antibody 67634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,CoIP

Reprod Biol Endocrinol

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Authors - Gelin Huang
View Article
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