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验证数据展示

  • WB analysis using 81527-1-RR (same clone as 81527-1-PBS)

    WB analysis using 81527-1-RR (same clone as 81527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81527-1-RR (same clone as 81527-1-PBS)

    WB analysis of HepG2 using 81527-1-RR (same clone as 81527-1-PBS)

    WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 81527-1-RR (same clone as 81527-1-PBS)

    IHC staining of mouse testis using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTK1 antibody included
  • IF Staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

    IF Staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81527-1-RR (same clone as 81527-1-PBS)

    IF Staining of HeLa using 81527-1-RR (same clone as 81527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GSTK1 Recombinant monoclonal antibody, PBS Only

GSTK1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 81527-1-PBS

产品说明书

CloneNo. 3J1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

DsbA-L, EC:2.5.1.18, Glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, GST

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSTK1 Ready-To-Use IHC Kit. GSTK1 primary antibody included.

产品信息

81527-1-PBS targets GSTK1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6036

Product name: Recombinant human GSTK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC050715

Sequence: MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione S-transferase kappa 1
别名 DsbA-L, EC:2.5.1.18, Glutathione S-transferase kappa 1, Glutathione S-transferase subunit 13, GST
计算分子量 32 kDa
观测分子量27 kDa
GenBank蛋白编号BC050715
基因名称 GSTK1
Gene ID (NCBI) 373156
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2Q3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

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