验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue, human testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
16703-1-AP targets GSTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10101 Product name: Recombinant human GSTA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC020619 Sequence: MAGKPKLHYFNGRGRMEPIRWLLAAAGVEFEEKFIGSAEDLGKLRNDGSLMFQQVPMVEIDGIKLVQTRAILNYIASKYNLYGKDIKERALIDMYTEGMADLNEMILLLPLCRPEEKDAKIALIKEKTKSRYFPAFEKVLQSHGQDYLVGNKLSRADISLVELLYYVEELDSSLISNFPLLKALKTRISNLPTVKKFLQPGSPRKPPADAKALEEARKIFRF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione S-transferase alpha 3 |
| 别名 | GST class-alpha member 3, Glutathione S-transferase A3-3, Glutathione S-transferase A3, Glutathione S transferase A3 3, Glutathione S transferase A3 |
| 计算分子量 | 222 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC020619 |
| 基因名称 | GSTA3 |
| Gene ID (NCBI) | 2940 |
| RRID | AB_2279523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16772 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| IP protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| WB protocol for GSTA3 antibody 16703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization. | ||
Am J Pathol Novel Protective Role of Nicotinamide Phosphoribosyltransferase in Acetaminophen-Induced Acute Liver Injury in Mice. | ||
PLoS One Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells. | ||
J Toxicol Pathol Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice. | ||
Radiother Oncol Transcriptomic and epigenetic landscape of nimorazole-enhanced radiochemotherapy in head and neck cancer |
