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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80006-1-RR

    WB analysis using 80006-1-RR

    GST tagged protein, target band 42 kDa and degraded band 32 kDa, and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Recombinant protein using 80006-1-RR

    IP experiment of Recombinant protein using 80006-1-RR

    IP result of anti-GST Tag (IP:80006-1-RR, 4ug; Detection:80006-1-RR 1:5000) with Recombinant protein lysate 1200 ug.

GST Tag Recombinant antibody

GST Tag Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 80006-1-RR

产品说明书

CloneNo. 9B24
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, IP, ELISA and More (1)

别名

GST, Glutathione S-Transferase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein
Positive IP detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80006-1-RR targets GST Tag in WB, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 recombinant protein
免疫原 GST Tag fusion protein Ag0040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GST Tag
别名 GST, Glutathione S-Transferase
计算分子量 26 kDa
GenBank蛋白编号FN315687
基因名称
Gene ID (NCBI)
RRIDAB_2882936
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.

实验方案

Product Specific Protocols
WB protocol for GST Tag antibody 80006-1-RRDownload protocol
IP protocol for GST Tag antibody 80006-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

bioRxiv

The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells

Authors - Michael R Weaver
View Article
CoIP

Vet Microbiol

PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication

Authors - Huanjie Zhai
View Article