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验证数据展示

  • WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

  • WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

  • IP experiment of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    IP experiment of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    IP result of anti-GST Tag (IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

GST Tag Monoclonal antibody, PBS Only

GST Tag Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 66001-2-PBS

产品说明书

CloneNo. 3G12B10

宿主/亚型

Mouse / IgG2a

种属反应性

Recombinant protein, Schistosoma japonicum

应用

WB, IP, Indirect ELISA

别名

GST, GST Tag

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66001-2-PBS targets GST Tag in WB, IP, Indirect ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 Recombinant protein, Schistosoma japonicum
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GST Tag
别名 GST, GST Tag
计算分子量 26 kDa
GenBank蛋白编号FN315687
基因名称
Gene ID (NCBI)
RRIDAB_2881488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.