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验证数据展示

  • WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66193-1-Ig (same clone as 66193-1-PBS)

    IHC staining of mouse testis using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GSNOR,ADH5 Monoclonal antibody, PBS Only

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66193-1-PBS

产品说明书

CloneNo. 5E6E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ADH5, 5E6E2, ADH 3, ADH 5, ADH3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66193-1-PBS targets GSNOR,ADH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag9511

Product name: Recombinant human GSNOR,ADH5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC014665

Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alcohol dehydrogenase 5 (class III), chi polypeptide
别名 ADH5, 5E6E2, ADH 3, ADH 5, ADH3
计算分子量 374 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC014665
基因名称 ADH5
Gene ID (NCBI) 128
RRIDAB_2881587
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP11766
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

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